Coding

Part:BBa_K2130002:Design

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-18)


Cas9 mutant 459


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
    Illegal BglII site found at 1647
    Illegal BglII site found at 1736
    Illegal BglII site found at 2381
    Illegal BamHI site found at 4105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

Addgene, the plasmid is then randomly mutated with EZClone Random mutation kit

References